Recombinant and in Vitro RNA Synthesis - (Methods in Molecular Biology) by Graeme L Conn (Hardcover)
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<p/><br></br><p><b> About the Book </b></p></br></br><p>Part of Springer's Methods in Molecular Biology series, this collection of detailed protocols guides the reader from the start of a new RNA project to the final sample, with step-by-step protocols and expert tips on deploying catalytic RNAs in the laboratory.</p><p/><br></br><p><b> Book Synopsis </b></p></br></br><p>1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold </p><p> </p><p>Luc Ponchon and Frédéric Dardel </p><p> </p><p> </p><p> </p><p>2. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold</p><p> </p><p>Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte and Frédéric Dardel </p><p> </p><p> </p><p> </p><p>3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR</p><p> </p><p>Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams</p><p> </p><p> </p><p> </p><p>4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE</p><p> </p><p>Jo L. Linpinsel and Graeme L. Conn</p><p> </p><p> </p><p> </p><p>5. Preparation of Short RNA by in vitro Transcription</p><p> </p><p>Cheng Lu and Pingwei Li</p><p> </p><p> </p><p> </p><p>6. Native RNA Purification by Gel Filtration Chromatography</p><p> </p><p>Evan P. Booy, Hui Meng and Sean A. McKenna</p><p> </p><p> </p><p>7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends</p><p> </p><p>Johanna M. Avis, Graeme L. Conn, and Scott C. Walker</p><p> </p><p> </p><p> </p><p>8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends </p><p> </p><p>Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka</p><p> </p><p> </p><p> </p><p>9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods </p><p> </p><p>Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong</p><p> </p><p> </p><p> </p><p>10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA</p><p> </p><p>Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault</p><p> </p><p> </p><p> </p><p>11. Affinity Purification of RNA using an ARiBo Tag</p><p> </p><p>Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompré-Brodeur, Vanessa Delfosse and Pascale Legault</p><p> </p><p> </p><p> </p><p>12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments</p><p> </p><p>Virginia K. Vachon and Graeme L. Conn</p><p> </p><p> </p><p> </p><p>13. In Vitro Transcription of Modified RNAs</p><p> </p><p>Stephanie L. Moon and Jeffrey Wilusz</p><p> </p><p> </p><p> </p><p>14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis</p><p> </p><p>N. Ruth Zearfoss and Sean P. Ryder</p><p> </p><p> </p><p> </p><p>15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA</p><p> </p><p>Ya-Ming Hou</p><p> </p><p> </p><p> </p><p>16. Se-derivatized RNAs for X-ray Crystallography</p><p> </p><p>Lina Lin and Zhen Huang</p><p> </p><p> </p><p> </p><p>17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs</p><p> </p><p>Luigi Martino and Maria R Conte</p><p> </p><p> </p><p> </p><p>18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC</p><p> </p><p>Pierre Dagenais and Pascale Legault</p><p> </p><p>19. Splint Ligation of RNA with T4 DNA Ligase</p><p> </p><p>Christopher J. Kershaw and Raymond T. O'Keefe</p><p/><br></br><p><b> From the Back Cover </b></p></br></br><p>The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In<i> </i><i>Recombinant and In Vitro RNA Synthesis: Methods and Protocols</i>, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful <i>Methods in Molecular Biology(TM)</i> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.</p><p> </p><p>Authoritative and practical, <i>Recombinant and In Vitro RNA Synthesis: Methods and Protocols</i> aids scientists in continuing to study the fascinating world of RNA.</p>
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